***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
28 V L -1.34 5.0 60
28 V I -0.93 5.0 60
28 V M -2.23 5.0 60
28 V F -2.85 5.0 60
28 V W -2.12 5.0 60
28 V Y -2.32 5.0 60
28 V G -3.73 5.0 60
28 V A -1.78 5.0 60
28 V P -2.88 5.0 60
28 V S -3.04 5.0 60
28 V T -2.17 5.0 60
28 V C -1.76 5.0 60
28 V H -2.10 5.0 60
28 V R -2.98 5.0 60
28 V K -2.46 5.0 60
28 V Q -2.27 5.0 60
28 V E -2.08 5.0 60
28 V N -3.14 5.0 60
28 V D -2.28 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************