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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
29 I V -0.48 5.0 60
29 I L 0.66 5.0 60
29 I M -0.95 5.0 60
29 I F -1.22 5.0 60
29 I W -1.99 5.0 60
29 I Y -2.23 5.0 60
29 I G -2.95 5.0 60
29 I A -2.53 5.0 60
29 I P -1.57 5.0 60
29 I S -2.55 5.0 60
29 I T -1.92 5.0 60
29 I C -2.80 5.0 60
29 I H -1.46 5.0 60
29 I R -2.23 5.0 60
29 I K -1.72 5.0 60
29 I Q -1.71 5.0 60
29 I E -1.20 5.0 60
29 I N -0.39 5.0 60
29 I D -2.18 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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