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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
30 A V 0.24 5.0 60
30 A L 0.41 5.0 60
30 A I 0.25 5.0 60
30 A M -0.01 5.0 60
30 A F 0.05 5.0 60
30 A W -0.37 5.0 60
30 A Y -0.31 5.0 60
30 A G -0.38 5.0 60
30 A P -0.05 5.0 60
30 A S -1.00 5.0 60
30 A T -0.40 5.0 60
30 A C -0.48 5.0 60
30 A H -0.33 5.0 60
30 A R -0.65 5.0 60
30 A K -0.33 5.0 60
30 A Q -0.46 5.0 60
30 A E -0.23 5.0 60
30 A N -0.35 5.0 60
30 A D -0.42 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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