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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
450 Q V 0.76 5.0 60
450 Q L 0.32 5.0 60
450 Q I 1.06 5.0 60
450 Q M -0.59 5.0 60
450 Q F 0.29 5.0 60
450 Q W -0.02 5.0 60
450 Q Y 0.48 5.0 60
450 Q G 0.01 5.0 60
450 Q A 0.23 5.0 60
450 Q P -1.21 5.0 60
450 Q S -0.89 5.0 60
450 Q T -0.86 5.0 60
450 Q C 0.90 5.0 60
450 Q H -0.93 5.0 60
450 Q R 0.13 5.0 60
450 Q K -0.45 5.0 60
450 Q E 0.29 5.0 60
450 Q N -1.99 5.0 60
450 Q D -0.35 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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