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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
31 G V 0.06 5.0 60
31 G L 0.08 5.0 60
31 G I 0.22 5.0 60
31 G M -0.36 5.0 60
31 G F -1.04 5.0 60
31 G W -0.75 5.0 60
31 G Y -0.95 5.0 60
31 G A -0.12 5.0 60
31 G P -0.07 5.0 60
31 G S -1.32 5.0 60
31 G T -1.23 5.0 60
31 G C -0.75 5.0 60
31 G H -0.64 5.0 60
31 G R -1.44 5.0 60
31 G K -0.75 5.0 60
31 G Q -0.28 5.0 60
31 G E 0.41 5.0 60
31 G N -0.41 5.0 60
31 G D -0.97 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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