***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
251 L V -1.50 7.0 25
251 L I -1.37 7.0 25
251 L M -1.51 7.0 25
251 L F -0.54 7.0 25
251 L W -0.50 7.0 25
251 L Y -0.92 7.0 25
251 L G -5.16 7.0 25
251 L A -4.44 7.0 25
251 L P -1.86 7.0 25
251 L S -4.56 7.0 25
251 L T -4.33 7.0 25
251 L C -4.25 7.0 25
251 L H -2.43 7.0 25
251 L R -2.85 7.0 25
251 L K -3.08 7.0 25
251 L Q -2.48 7.0 25
251 L E -1.97 7.0 25
251 L N -2.99 7.0 25
251 L D -3.84 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************