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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
32 G V 0.24 5.0 60
32 G L 0.24 5.0 60
32 G I 0.29 5.0 60
32 G M -0.43 5.0 60
32 G F -1.26 5.0 60
32 G W -0.95 5.0 60
32 G Y -1.10 5.0 60
32 G A -0.36 5.0 60
32 G P -0.15 5.0 60
32 G S -1.53 5.0 60
32 G T -1.52 5.0 60
32 G C -0.69 5.0 60
32 G H -0.94 5.0 60
32 G R -1.50 5.0 60
32 G K -0.95 5.0 60
32 G Q -0.31 5.0 60
32 G E 0.17 5.0 60
32 G N -0.60 5.0 60
32 G D -1.31 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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