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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
19 K V 0.40 5.0 60
19 K L 0.27 5.0 60
19 K I 0.65 5.0 60
19 K M -0.36 5.0 60
19 K F 1.28 5.0 60
19 K W -0.30 5.0 60
19 K Y 0.45 5.0 60
19 K G -1.11 5.0 60
19 K A -0.70 5.0 60
19 K P 0.73 5.0 60
19 K S -1.08 5.0 60
19 K T -0.66 5.0 60
19 K C -0.32 5.0 60
19 K H -0.89 5.0 60
19 K R -0.47 5.0 60
19 K Q -0.88 5.0 60
19 K E -0.50 5.0 60
19 K N -0.94 5.0 60
19 K D -1.10 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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