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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
34 L V -1.92 5.0 60
34 L I -1.60 5.0 60
34 L M -1.92 5.0 60
34 L F -2.21 5.0 60
34 L W -1.87 5.0 60
34 L Y -2.03 5.0 60
34 L G -5.01 5.0 60
34 L A -4.02 5.0 60
34 L P -2.31 5.0 60
34 L S -4.16 5.0 60
34 L T -4.31 5.0 60
34 L C -4.09 5.0 60
34 L H -2.36 5.0 60
34 L R -3.82 5.0 60
34 L K -3.59 5.0 60
34 L Q -2.54 5.0 60
34 L E -2.26 5.0 60
34 L N -3.37 5.0 60
34 L D -4.43 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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