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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
89 T V -0.21 7.0 25
89 T L -0.37 7.0 25
89 T I -0.42 7.0 25
89 T M 0.23 7.0 25
89 T F 0.51 7.0 25
89 T W 0.13 7.0 25
89 T Y 1.27 7.0 25
89 T G -2.02 7.0 25
89 T A -0.87 7.0 25
89 T P -1.69 7.0 25
89 T S -0.87 7.0 25
89 T C -0.17 7.0 25
89 T H -1.41 7.0 25
89 T R 0.68 7.0 25
89 T K -0.75 7.0 25
89 T Q -1.15 7.0 25
89 T E -0.05 7.0 25
89 T N -0.20 7.0 25
89 T D -0.29 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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