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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
456 K V 0.82 5.0 60
456 K L -0.11 5.0 60
456 K I 0.55 5.0 60
456 K M 0.08 5.0 60
456 K F 0.95 5.0 60
456 K W -0.70 5.0 60
456 K Y 0.48 5.0 60
456 K G 0.10 5.0 60
456 K A -0.29 5.0 60
456 K P -0.00 5.0 60
456 K S -0.84 5.0 60
456 K T -1.32 5.0 60
456 K C 0.06 5.0 60
456 K H -0.96 5.0 60
456 K R 0.27 5.0 60
456 K Q -0.34 5.0 60
456 K E -0.72 5.0 60
456 K N -0.37 5.0 60
456 K D -1.24 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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