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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
157 V L -0.31 7.0 25
157 V I -0.31 7.0 25
157 V M -0.32 7.0 25
157 V F -0.87 7.0 25
157 V W -0.69 7.0 25
157 V Y -0.89 7.0 25
157 V G -2.15 7.0 25
157 V A -1.27 7.0 25
157 V P -1.96 7.0 25
157 V S -1.28 7.0 25
157 V T -0.62 7.0 25
157 V C 0.27 7.0 25
157 V H -2.08 7.0 25
157 V R -1.05 7.0 25
157 V K -1.57 7.0 25
157 V Q -1.19 7.0 25
157 V E -1.03 7.0 25
157 V N -1.50 7.0 25
157 V D -1.54 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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