***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
458 F V -2.24 5.0 60
458 F L -2.00 5.0 60
458 F I -0.67 5.0 60
458 F M -0.94 5.0 60
458 F W -0.28 5.0 60
458 F Y -0.37 5.0 60
458 F G -2.56 5.0 60
458 F A -1.62 5.0 60
458 F P -2.68 5.0 60
458 F S -2.36 5.0 60
458 F T -2.06 5.0 60
458 F C -1.19 5.0 60
458 F H -2.62 5.0 60
458 F R -1.16 5.0 60
458 F K -1.60 5.0 60
458 F Q -1.60 5.0 60
458 F E -1.27 5.0 60
458 F N -1.85 5.0 60
458 F D -2.31 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************