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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
459 M V -0.10 5.0 60
459 M L -0.56 5.0 60
459 M I -0.11 5.0 60
459 M F 0.29 5.0 60
459 M W -0.40 5.0 60
459 M Y -0.03 5.0 60
459 M G -1.63 5.0 60
459 M A -0.76 5.0 60
459 M P -1.85 5.0 60
459 M S -1.20 5.0 60
459 M T -1.16 5.0 60
459 M C 0.23 5.0 60
459 M H -1.39 5.0 60
459 M R -0.40 5.0 60
459 M K -1.47 5.0 60
459 M Q -0.67 5.0 60
459 M E -0.26 5.0 60
459 M N -0.40 5.0 60
459 M D -0.43 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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