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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
471 T V 0.64 5.0 60
471 T L -0.19 5.0 60
471 T I 0.09 5.0 60
471 T M -0.24 5.0 60
471 T F -0.56 5.0 60
471 T W 0.03 5.0 60
471 T Y 0.49 5.0 60
471 T G -1.02 5.0 60
471 T A -0.13 5.0 60
471 T P -0.53 5.0 60
471 T S 0.19 5.0 60
471 T C 0.14 5.0 60
471 T H -0.75 5.0 60
471 T R 0.20 5.0 60
471 T K -0.75 5.0 60
471 T Q -0.40 5.0 60
471 T E 0.61 5.0 60
471 T N -0.20 5.0 60
471 T D 0.41 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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