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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
10 R V -0.64 7.0 25
10 R L -0.52 7.0 25
10 R I -0.42 7.0 25
10 R M -1.27 7.0 25
10 R F -0.60 7.0 25
10 R W -0.87 7.0 25
10 R Y -0.69 7.0 25
10 R G -1.59 7.0 25
10 R A -0.57 7.0 25
10 R P -0.99 7.0 25
10 R S -2.30 7.0 25
10 R T -1.54 7.0 25
10 R C -0.28 7.0 25
10 R H -1.55 7.0 25
10 R K -1.31 7.0 25
10 R Q -1.41 7.0 25
10 R E -0.69 7.0 25
10 R N -1.71 7.0 25
10 R D -1.40 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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