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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
482 E V 0.56 5.0 60
482 E L 0.86 5.0 60
482 E I 1.32 5.0 60
482 E M 0.53 5.0 60
482 E F 1.07 5.0 60
482 E W 0.17 5.0 60
482 E Y 0.65 5.0 60
482 E G -1.49 5.0 60
482 E A -0.01 5.0 60
482 E P 0.35 5.0 60
482 E S -0.25 5.0 60
482 E T 0.28 5.0 60
482 E C 0.29 5.0 60
482 E H 0.06 5.0 60
482 E R 0.03 5.0 60
482 E K -0.59 5.0 60
482 E Q 0.20 5.0 60
482 E N -0.66 5.0 60
482 E D -0.10 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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