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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
252 T V -0.34 7.0 25
252 T L -1.32 7.0 25
252 T I -0.73 7.0 25
252 T M -0.73 7.0 25
252 T F 0.08 7.0 25
252 T W -0.07 7.0 25
252 T Y 0.43 7.0 25
252 T G -1.95 7.0 25
252 T A -1.24 7.0 25
252 T P -1.32 7.0 25
252 T S -1.50 7.0 25
252 T C -1.75 7.0 25
252 T H -2.03 7.0 25
252 T R -0.37 7.0 25
252 T K -0.93 7.0 25
252 T Q -1.47 7.0 25
252 T E -0.06 7.0 25
252 T N -0.69 7.0 25
252 T D 0.08 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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