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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
36 G V -0.11 5.0 60
36 G L -0.27 5.0 60
36 G I 0.05 5.0 60
36 G M -0.99 5.0 60
36 G F -1.76 5.0 60
36 G W -1.26 5.0 60
36 G Y -1.59 5.0 60
36 G A -0.49 5.0 60
36 G P -0.35 5.0 60
36 G S -1.39 5.0 60
36 G T -1.79 5.0 60
36 G C -1.02 5.0 60
36 G H -1.43 5.0 60
36 G R -1.87 5.0 60
36 G K -1.55 5.0 60
36 G Q -0.59 5.0 60
36 G E 0.27 5.0 60
36 G N -1.18 5.0 60
36 G D -1.34 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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