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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
498 P V -0.31 5.0 60
498 P L 0.57 5.0 60
498 P I 0.42 5.0 60
498 P M 0.06 5.0 60
498 P F 0.20 5.0 60
498 P W -0.35 5.0 60
498 P Y -0.62 5.0 60
498 P G 0.38 5.0 60
498 P A -0.58 5.0 60
498 P S -0.82 5.0 60
498 P T -0.71 5.0 60
498 P C 0.34 5.0 60
498 P H -0.59 5.0 60
498 P R -0.28 5.0 60
498 P K -0.10 5.0 60
498 P Q -0.70 5.0 60
498 P E -0.06 5.0 60
498 P N -1.05 5.0 60
498 P D -1.15 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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