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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
499 Q V 0.33 5.0 60
499 Q L 0.81 5.0 60
499 Q I 0.99 5.0 60
499 Q M -0.43 5.0 60
499 Q F 0.50 5.0 60
499 Q W 0.23 5.0 60
499 Q Y 0.55 5.0 60
499 Q G 0.94 5.0 60
499 Q A 0.34 5.0 60
499 Q P -1.41 5.0 60
499 Q S -0.67 5.0 60
499 Q T -0.29 5.0 60
499 Q C 1.39 5.0 60
499 Q H -0.73 5.0 60
499 Q R -0.41 5.0 60
499 Q K 0.45 5.0 60
499 Q E 0.54 5.0 60
499 Q N -1.94 5.0 60
499 Q D -0.59 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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