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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
38 T V 0.27 5.0 60
38 T L -0.24 5.0 60
38 T I -0.39 5.0 60
38 T M -0.20 5.0 60
38 T F -0.62 5.0 60
38 T W -0.21 5.0 60
38 T Y 0.48 5.0 60
38 T G -1.05 5.0 60
38 T A -0.26 5.0 60
38 T P -0.99 5.0 60
38 T S 0.25 5.0 60
38 T C 0.06 5.0 60
38 T H -0.58 5.0 60
38 T R -0.21 5.0 60
38 T K -0.62 5.0 60
38 T Q -0.71 5.0 60
38 T E 0.55 5.0 60
38 T N -0.22 5.0 60
38 T D 0.37 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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