***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
39 V L -1.48 5.0 60
39 V I -0.81 5.0 60
39 V M -2.90 5.0 60
39 V F -3.31 5.0 60
39 V W -2.27 5.0 60
39 V Y -2.57 5.0 60
39 V G -4.06 5.0 60
39 V A -1.88 5.0 60
39 V P -2.94 5.0 60
39 V S -2.80 5.0 60
39 V T -1.96 5.0 60
39 V C -1.56 5.0 60
39 V H -1.89 5.0 60
39 V R -2.77 5.0 60
39 V K -2.73 5.0 60
39 V Q -2.08 5.0 60
39 V E -1.72 5.0 60
39 V N -3.17 5.0 60
39 V D -2.15 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************