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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
40 A V 0.37 5.0 60
40 A L 0.20 5.0 60
40 A I 0.15 5.0 60
40 A M -0.53 5.0 60
40 A F -0.39 5.0 60
40 A W -0.63 5.0 60
40 A Y -0.97 5.0 60
40 A G -0.46 5.0 60
40 A P -0.51 5.0 60
40 A S -0.54 5.0 60
40 A T -0.17 5.0 60
40 A C -0.47 5.0 60
40 A H -0.54 5.0 60
40 A R -0.65 5.0 60
40 A K -1.14 5.0 60
40 A Q -0.45 5.0 60
40 A E 0.07 5.0 60
40 A N -0.86 5.0 60
40 A D -0.00 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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