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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
529 R V -0.19 5.0 60
529 R L 0.25 5.0 60
529 R I 0.21 5.0 60
529 R M -0.48 5.0 60
529 R F -0.26 5.0 60
529 R W -0.38 5.0 60
529 R Y -0.81 5.0 60
529 R G -1.91 5.0 60
529 R A -0.25 5.0 60
529 R P -0.52 5.0 60
529 R S -2.36 5.0 60
529 R T -0.69 5.0 60
529 R C -0.22 5.0 60
529 R H -1.10 5.0 60
529 R K -1.62 5.0 60
529 R Q -1.05 5.0 60
529 R E -0.73 5.0 60
529 R N -1.40 5.0 60
529 R D -0.97 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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