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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
41 A V 0.38 5.0 60
41 A L 0.13 5.0 60
41 A I 0.18 5.0 60
41 A M -0.55 5.0 60
41 A F -0.30 5.0 60
41 A W -0.64 5.0 60
41 A Y -1.01 5.0 60
41 A G -0.50 5.0 60
41 A P -0.71 5.0 60
41 A S -0.56 5.0 60
41 A T 0.02 5.0 60
41 A C -0.50 5.0 60
41 A H -0.81 5.0 60
41 A R -0.62 5.0 60
41 A K -1.35 5.0 60
41 A Q -0.44 5.0 60
41 A E 0.04 5.0 60
41 A N -0.94 5.0 60
41 A D -0.02 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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