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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
1 S V 0.56 7.0 25
1 S L 0.30 7.0 25
1 S I 0.68 7.0 25
1 S M 0.04 7.0 25
1 S F 0.75 7.0 25
1 S W 0.16 7.0 25
1 S Y 0.18 7.0 25
1 S G -1.61 7.0 25
1 S A 0.12 7.0 25
1 S P -0.41 7.0 25
1 S T -0.54 7.0 25
1 S C -0.55 7.0 25
1 S H -0.36 7.0 25
1 S R 0.47 7.0 25
1 S K 0.50 7.0 25
1 S Q -0.46 7.0 25
1 S E 0.08 7.0 25
1 S N 0.13 7.0 25
1 S D -0.10 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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