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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
702 N V 0.89 7.0 25
702 N L 0.79 7.0 25
702 N I 1.43 7.0 25
702 N M 0.65 7.0 25
702 N F 1.03 7.0 25
702 N W 0.14 7.0 25
702 N Y 0.01 7.0 25
702 N G -2.61 7.0 25
702 N A -0.80 7.0 25
702 N P -0.62 7.0 25
702 N S -0.66 7.0 25
702 N T -0.47 7.0 25
702 N C -0.39 7.0 25
702 N H -2.00 7.0 25
702 N R -0.25 7.0 25
702 N K -0.72 7.0 25
702 N Q -1.29 7.0 25
702 N E -0.08 7.0 25
702 N D -1.18 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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