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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
1 H V 1.20 7.0 25
1 H L 1.80 7.0 25
1 H I 1.74 7.0 25
1 H M 1.01 7.0 25
1 H F 1.40 7.0 25
1 H W 0.80 7.0 25
1 H Y 1.79 7.0 25
1 H G 0.32 7.0 25
1 H A 0.65 7.0 25
1 H P 0.95 7.0 25
1 H S 0.90 7.0 25
1 H T 0.09 7.0 25
1 H C 1.25 7.0 25
1 H R 0.97 7.0 25
1 H K 0.90 7.0 25
1 H Q 0.01 7.0 25
1 H E 0.91 7.0 25
1 H N -0.90 7.0 25
1 H D -0.19 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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