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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
183 G V -0.03 5.0 60
183 G L -0.15 5.0 60
183 G I 0.14 5.0 60
183 G M -0.93 5.0 60
183 G F -0.59 5.0 60
183 G W -0.61 5.0 60
183 G Y -1.11 5.0 60
183 G A 0.20 5.0 60
183 G P -0.45 5.0 60
183 G S -0.72 5.0 60
183 G T -1.27 5.0 60
183 G C 0.08 5.0 60
183 G H -1.10 5.0 60
183 G R -0.40 5.0 60
183 G K -0.96 5.0 60
183 G Q -0.67 5.0 60
183 G E 0.35 5.0 60
183 G N -1.11 5.0 60
183 G D -0.90 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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