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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
539 A V 0.11 5.0 60
539 A L 0.19 5.0 60
539 A I 0.14 5.0 60
539 A M -0.50 5.0 60
539 A F -0.13 5.0 60
539 A W -0.51 5.0 60
539 A Y -0.86 5.0 60
539 A G -1.18 5.0 60
539 A P -0.74 5.0 60
539 A S -1.29 5.0 60
539 A T -0.49 5.0 60
539 A C -0.90 5.0 60
539 A H -1.17 5.0 60
539 A R -0.98 5.0 60
539 A K -0.96 5.0 60
539 A Q -0.75 5.0 60
539 A E -0.45 5.0 60
539 A N -0.47 5.0 60
539 A D -0.65 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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