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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
19 A V 1.03 7.0 25
19 A L 0.32 7.0 25
19 A I 0.56 7.0 25
19 A M -0.08 7.0 25
19 A F 0.12 7.0 25
19 A W -0.73 7.0 25
19 A Y -1.07 7.0 25
19 A G -1.37 7.0 25
19 A P -0.42 7.0 25
19 A S -0.43 7.0 25
19 A T -1.05 7.0 25
19 A C 0.20 7.0 25
19 A H -1.29 7.0 25
19 A R 0.08 7.0 25
19 A K -1.30 7.0 25
19 A Q -0.83 7.0 25
19 A E -0.81 7.0 25
19 A N -1.40 7.0 25
19 A D -0.65 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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