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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
148 I V -1.40 7.0 25
148 I L -0.70 7.0 25
148 I M -1.91 7.0 25
148 I F -1.94 7.0 25
148 I W -3.04 7.0 25
148 I Y -3.26 7.0 25
148 I G -4.89 7.0 25
148 I A -3.98 7.0 25
148 I P -2.87 7.0 25
148 I S -3.76 7.0 25
148 I T -3.68 7.0 25
148 I C -4.27 7.0 25
148 I H -2.86 7.0 25
148 I R -2.60 7.0 25
148 I K -3.37 7.0 25
148 I Q -3.07 7.0 25
148 I E -2.53 7.0 25
148 I N -1.72 7.0 25
148 I D -3.09 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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