***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
59 S V 0.06 7.0 25
59 S L 0.14 7.0 25
59 S I 0.19 7.0 25
59 S M -0.26 7.0 25
59 S F 0.89 7.0 25
59 S W 0.07 7.0 25
59 S Y 0.35 7.0 25
59 S G -1.23 7.0 25
59 S A -0.23 7.0 25
59 S P -0.97 7.0 25
59 S T -0.78 7.0 25
59 S C -0.78 7.0 25
59 S H -0.40 7.0 25
59 S R -0.08 7.0 25
59 S K 0.49 7.0 25
59 S Q -0.61 7.0 25
59 S E 0.06 7.0 25
59 S N -0.12 7.0 25
59 S D -0.15 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************