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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
20 V L -1.35 5.0 60
20 V I -0.28 5.0 60
20 V M -2.38 5.0 60
20 V F -2.78 5.0 60
20 V W -2.04 5.0 60
20 V Y -2.24 5.0 60
20 V G -4.32 5.0 60
20 V A -2.40 5.0 60
20 V P -3.07 5.0 60
20 V S -3.29 5.0 60
20 V T -2.59 5.0 60
20 V C -1.58 5.0 60
20 V H -2.78 5.0 60
20 V R -2.72 5.0 60
20 V K -2.70 5.0 60
20 V Q -2.74 5.0 60
20 V E -2.10 5.0 60
20 V N -4.36 5.0 60
20 V D -2.59 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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