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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
253 G V 0.54 7.0 25
253 G L -0.50 7.0 25
253 G I 0.48 7.0 25
253 G M -0.47 7.0 25
253 G F 0.01 7.0 25
253 G W 0.10 7.0 25
253 G Y -0.58 7.0 25
253 G A -0.09 7.0 25
253 G P 0.12 7.0 25
253 G S -1.31 7.0 25
253 G T -1.35 7.0 25
253 G C -1.05 7.0 25
253 G H -1.38 7.0 25
253 G R -0.83 7.0 25
253 G K -0.77 7.0 25
253 G Q -0.22 7.0 25
253 G E 0.91 7.0 25
253 G N -0.19 7.0 25
253 G D -0.30 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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