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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
196 T V 0.83 5.0 60
196 T L -0.00 5.0 60
196 T I -0.23 5.0 60
196 T M -0.31 5.0 60
196 T F 0.16 5.0 60
196 T W -0.04 5.0 60
196 T Y 0.24 5.0 60
196 T G -2.62 5.0 60
196 T A -0.50 5.0 60
196 T P -1.14 5.0 60
196 T S -0.83 5.0 60
196 T C 0.02 5.0 60
196 T H -1.91 5.0 60
196 T R 0.07 5.0 60
196 T K -1.02 5.0 60
196 T Q -1.44 5.0 60
196 T E -0.17 5.0 60
196 T N -0.55 5.0 60
196 T D 0.17 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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