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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
235 E V 0.94 5.0 60
235 E L 0.85 5.0 60
235 E I 1.38 5.0 60
235 E M 0.73 5.0 60
235 E F 1.05 5.0 60
235 E W 0.42 5.0 60
235 E Y 0.26 5.0 60
235 E G 0.34 5.0 60
235 E A 0.32 5.0 60
235 E P 0.01 5.0 60
235 E S -0.17 5.0 60
235 E T 0.17 5.0 60
235 E C 1.09 5.0 60
235 E H -0.33 5.0 60
235 E R 0.99 5.0 60
235 E K -0.77 5.0 60
235 E Q 0.04 5.0 60
235 E N -0.25 5.0 60
235 E D -0.08 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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