***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
300 L V -1.27 5.0 60
300 L I -0.94 5.0 60
300 L M -1.00 5.0 60
300 L F -1.96 5.0 60
300 L W -1.23 5.0 60
300 L Y -1.60 5.0 60
300 L G -3.48 5.0 60
300 L A -2.77 5.0 60
300 L P -1.09 5.0 60
300 L S -2.48 5.0 60
300 L T -3.60 5.0 60
300 L C -2.39 5.0 60
300 L H -1.54 5.0 60
300 L R -2.66 5.0 60
300 L K -2.87 5.0 60
300 L Q -1.87 5.0 60
300 L E -1.55 5.0 60
300 L N -3.13 5.0 60
300 L D -2.70 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************