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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
635 S V 1.27 7.0 25
635 S L 0.84 7.0 25
635 S I 1.12 7.0 25
635 S M -0.12 7.0 25
635 S F 0.16 7.0 25
635 S W 0.54 7.0 25
635 S Y -0.22 7.0 25
635 S G -1.95 7.0 25
635 S A 0.17 7.0 25
635 S P 0.20 7.0 25
635 S T -0.64 7.0 25
635 S C -0.49 7.0 25
635 S H -0.48 7.0 25
635 S R -0.16 7.0 25
635 S K 0.15 7.0 25
635 S Q -0.07 7.0 25
635 S E 0.37 7.0 25
635 S N 0.14 7.0 25
635 S D 0.08 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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