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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
304 Y V 0.16 5.0 60
304 Y L 0.21 5.0 60
304 Y I 0.33 5.0 60
304 Y M -0.17 5.0 60
304 Y F -1.04 5.0 60
304 Y W 0.02 5.0 60
304 Y G -2.51 5.0 60
304 Y A -1.15 5.0 60
304 Y P -0.28 5.0 60
304 Y S -0.43 5.0 60
304 Y T -0.01 5.0 60
304 Y C 1.33 5.0 60
304 Y H -0.22 5.0 60
304 Y R -0.40 5.0 60
304 Y K -0.40 5.0 60
304 Y Q -0.20 5.0 60
304 Y E 0.23 5.0 60
304 Y N -1.48 5.0 60
304 Y D 0.87 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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