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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
391 N V 2.05 5.0 60
391 N L 2.76 5.0 60
391 N I 2.80 5.0 60
391 N M 2.14 5.0 60
391 N F 1.25 5.0 60
391 N W 0.80 5.0 60
391 N Y 0.98 5.0 60
391 N G -1.54 5.0 60
391 N A -0.03 5.0 60
391 N P 0.29 5.0 60
391 N S 0.27 5.0 60
391 N T 0.79 5.0 60
391 N C 0.76 5.0 60
391 N H -0.92 5.0 60
391 N R 0.08 5.0 60
391 N K -0.35 5.0 60
391 N Q 0.13 5.0 60
391 N E 0.59 5.0 60
391 N D -0.08 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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