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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
254 Q V 0.65 7.0 25
254 Q L -0.32 7.0 25
254 Q I 0.62 7.0 25
254 Q M -1.37 7.0 25
254 Q F 0.21 7.0 25
254 Q W 0.07 7.0 25
254 Q Y -0.11 7.0 25
254 Q G -1.72 7.0 25
254 Q A -0.68 7.0 25
254 Q P -0.55 7.0 25
254 Q S -1.60 7.0 25
254 Q T -1.16 7.0 25
254 Q C -0.61 7.0 25
254 Q H -1.28 7.0 25
254 Q R -1.36 7.0 25
254 Q K -0.41 7.0 25
254 Q E 0.41 7.0 25
254 Q N -1.85 7.0 25
254 Q D -0.29 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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