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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
1 Y V 0.51 5.0 60
1 Y L 1.25 5.0 60
1 Y I 0.75 5.0 60
1 Y M 0.56 5.0 60
1 Y F 0.74 5.0 60
1 Y W 0.96 5.0 60
1 Y G -2.61 5.0 60
1 Y A -0.92 5.0 60
1 Y P -0.59 5.0 60
1 Y S -0.37 5.0 60
1 Y T 0.53 5.0 60
1 Y C 2.15 5.0 60
1 Y H -0.24 5.0 60
1 Y R 0.62 5.0 60
1 Y K 0.48 5.0 60
1 Y Q 0.38 5.0 60
1 Y E 0.34 5.0 60
1 Y N -0.29 5.0 60
1 Y D 0.34 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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