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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
35 F V -1.88 7.0 25
35 F L -0.43 7.0 25
35 F I -0.89 7.0 25
35 F M -1.02 7.0 25
35 F W -0.22 7.0 25
35 F Y -0.38 7.0 25
35 F G -3.35 7.0 25
35 F A -2.49 7.0 25
35 F P -1.51 7.0 25
35 F S -1.79 7.0 25
35 F T -1.54 7.0 25
35 F C -1.04 7.0 25
35 F H -1.80 7.0 25
35 F R -1.31 7.0 25
35 F K -1.63 7.0 25
35 F Q -1.64 7.0 25
35 F E -1.06 7.0 25
35 F N -1.49 7.0 25
35 F D -1.67 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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