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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
2 S V 0.76 5.0 60
2 S L 0.76 5.0 60
2 S I 1.28 5.0 60
2 S M 0.67 5.0 60
2 S F 0.93 5.0 60
2 S W 0.73 5.0 60
2 S Y 0.23 5.0 60
2 S G -1.21 5.0 60
2 S A 0.71 5.0 60
2 S P -0.46 5.0 60
2 S T 0.26 5.0 60
2 S C -0.12 5.0 60
2 S H -0.54 5.0 60
2 S R 0.18 5.0 60
2 S K 0.60 5.0 60
2 S Q 0.03 5.0 60
2 S E 0.23 5.0 60
2 S N 0.45 5.0 60
2 S D 0.10 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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