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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
3 P V -0.89 5.0 60
3 P L 0.24 5.0 60
3 P I 0.05 5.0 60
3 P M -0.27 5.0 60
3 P F -0.20 5.0 60
3 P W -0.64 5.0 60
3 P Y -1.08 5.0 60
3 P G -1.93 5.0 60
3 P A -1.75 5.0 60
3 P S -1.16 5.0 60
3 P T -1.11 5.0 60
3 P C -0.41 5.0 60
3 P H -1.61 5.0 60
3 P R -0.38 5.0 60
3 P K -0.99 5.0 60
3 P Q -1.28 5.0 60
3 P E -0.85 5.0 60
3 P N -1.32 5.0 60
3 P D -1.40 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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