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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
2 W V -0.71 3.0 25
2 W L -0.23 3.0 25
2 W I -0.50 3.0 25
2 W M -0.92 3.0 25
2 W F -0.97 3.0 25
2 W Y -0.51 3.0 25
2 W G -2.51 3.0 25
2 W A -1.54 3.0 25
2 W P -1.26 3.0 25
2 W S -1.53 3.0 25
2 W T -1.47 3.0 25
2 W C -1.15 3.0 25
2 W H -2.36 3.0 25
2 W R -0.74 3.0 25
2 W K -1.31 3.0 25
2 W Q -1.53 3.0 25
2 W E -1.03 3.0 25
2 W N -1.58 3.0 25
2 W D -1.73 3.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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