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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
172 G V 0.14 7.0 25
172 G L 0.32 7.0 25
172 G I 0.19 7.0 25
172 G M 0.44 7.0 25
172 G F 0.22 7.0 25
172 G W -0.11 7.0 25
172 G Y -0.12 7.0 25
172 G A -0.43 7.0 25
172 G P -0.01 7.0 25
172 G S -0.44 7.0 25
172 G T -0.93 7.0 25
172 G C -0.11 7.0 25
172 G H -0.29 7.0 25
172 G R 0.16 7.0 25
172 G K -0.22 7.0 25
172 G Q 0.00 7.0 25
172 G E 0.58 7.0 25
172 G N 0.15 7.0 25
172 G D -0.30 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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