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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
173 V L -0.82 7.0 25
173 V I -1.01 7.0 25
173 V M -0.99 7.0 25
173 V F -1.60 7.0 25
173 V W -1.45 7.0 25
173 V Y -1.55 7.0 25
173 V G -4.26 7.0 25
173 V A -2.07 7.0 25
173 V P -2.32 7.0 25
173 V S -2.51 7.0 25
173 V T -1.76 7.0 25
173 V C -1.22 7.0 25
173 V H -1.58 7.0 25
173 V R -1.63 7.0 25
173 V K -2.01 7.0 25
173 V Q -1.98 7.0 25
173 V E -1.52 7.0 25
173 V N -2.10 7.0 25
173 V D -1.68 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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